Lipidure®-CM5206
－Features－
-Hemo-compatibility
-Anti-adhesion of proteins and cells
-Anti-denaturing of proteins
-Anti-activation of cells
－Usage－
-Dissolve Lipidure®-CM in ethanol and prepare a 0.5wt% ethanol solution.
-Dip the base material into the coating solution for 1 min.
-Dry the coated material at room temperature or one hour at 50ｰC.
－Substrates－
-Lipidure®-CM can be coated on the following substrates:
-Plastics (PET, PMMA, PSt, PU, PC, PE, PP)
-Glass, Ceramics and Metals (SUS, titanium, gold)
Lipidure-CM5206
m=3, n=7, Alkyl= n-Butyl
Lipidure-CM5206 is equivalent to “PMB30”
in the Professors Nobuo Nakabayashi’s and Kazuhiko Ishihara’s literatures.
References
K. Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 28, 1347-1355(1994)
K. Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 39, 323-330(1998)
K. Ishihara, N. Nakabayashi et al., J. Biomed. Mater. Res. 64, 411-416(2003)
N. Nakabayashi, Macromol. Symp. 245-246, 591-598(2006)
－Comparison of results－
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Experimental procedure
-Dispensation of HRP-IgG conjugate solution into each well
-Incubation for 1 h at 25°C
-Washing three times with Dulbeco’ PBS including 0.05% of Tween20.
-Dispensation of 3,3’,5,5’ - tetramethylbenzidine solution
-Measurement of absorbance at 450 nm
The Lipidure® treatment was able to reduce the amount of IgG absorption to below 10% of non-treated pleat. The same results were observed with other proteins.
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Experimental procedure
-Seeding of macrophage into each well (3.3 x 105 cells/well)
-Incubation for 1 h at 37°C in 5% CO2 atmosphere
-Washing with Dulbeco’ PBS
-Fixing with glutaraldehyde solution
-Observation by SEM
The activation and adhesion of macrophage was prevented by Lipidure® treatment. Also, the Lipidure® treatment was effective for other cells.
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－Coating－
PC surface can be made by coating with Lipidure®-CM. The following figure shows the relationship between “he number of times the coating was applied”and “he thickness of the coating film” The thickness of the Lipidure®-CM coating film is calculated by weight measurement. These data are examples in the case of dip coating. The thickness was controlled by adjusting the number of times of a coating was applied and the concentration of Lipidure® in the coating solution.
－Safety Data－
We have the following toxicological data for Lipidure®-CM5206. Acute oral toxicity, Skin irritation, Skin sensitization, Mutagenic potential (Ames Test), Hemolysis test, Endotoxin test
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